Be the First to Know About IntestiCult™ Plus Organoid Growth Medium
Redefine Your Intestinal Organoid Culture
Most commonly used intestinal organoids lack key differentiated cells, which can skew experimental results and limit research translatability. IntestiCult™ Plus Organoid Growth Medium (IntestiCult™ Plus) redefines intestinal organoid culture by enabling the growth of organoids that contain both proliferative stem cells and mature, functionally diverse intestinal cell types. Specifically formulated to balance support for both stem and differentiated cells to enhance biological fidelity, this medium ensures your organoids reflect the complexity of the in vivo human intestine. By supporting a physiologically balanced cellular composition, IntestiCult™ Plus gives you greater confidence in your experimental readouts—whether you’re modeling disease, evaluating drugs, or assessing toxicity.
Organoid Morphology and Specialized Cell Types That Reflect the Intestine
With IntestiCult™ Plus, organoids reliably exhibit region-specific budding and crypt-like structures while supporting the differentiation of key specialized intestinal cell types. This enables the generation of organoids that better reflect the native epithelial architecture compared to first-generation systems such as IntestiCult™ Organoid Growth Medium.
Figure 1. Organoids Cultured Using IntestiCult™ Plus Demonstrate Increased Budding and Crypt-Like Structures, Which Supports the Differentiation of Specialized Cell Types
(A) When expanded with first-generation expansion medium (IntestiCult™ Organoid Growth Medium), organoids from duodenal, ileum, and colonic tissues exhibit a thin-walled cystic morphology, as demonstrated by brightfield imaging. Scale bar = 250 µm. (B) In contrast, organoids expanded with IntestiCult™ Plus Organoid Growth Medium exhibit a complex budded morphology, with subtle yet consistent morphological differences between intestinal regions (duodenum, ileum, and colon). Scale bar = 250 µm. Confocal images of organoids from the same three tissue regions demonstrate the presence of specialized intestinal cell types when cultured with IntestiCult™ Plus, including (C) goblet cells (MUC2). Scale bar = 100 µm.
Why Use IntestiCult™ Plus?
- Support diverse cell types and achieve greater physiological relevance with organoids that model the full crypt-villus axis.
- Accelerate organoid production by simultaneously expanding and differentiating cultures, delivering assay-ready organoids sooner.
- Ensure reproducible results with a serum-free, conditioned medium-free formulation that minimizes variability.
- Skip the labor-intensive process of optimizing and making media based on published formulations.
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