Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaTox™ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCult™ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Hematopoietic Colony Enumeration and Identification: A Brief OverviewOverview of the different classes of hematopoietic progenitors detected in colony-forming unit (CFU) assays using various formulations of MethoCult™ methylcellulose-based media -
How to Set Up the CFU Assay for Custom Hematopoietic Training Courses Using SmartDish™Learn how to perform all steps involved in the colony-forming unit (CFU) assay setup for custom hematopoietic training courses using SmartDish™ cultureware -
CFU Assay Setup for Custom Hematopoietic Training CoursesLearn how to perform all steps involved in the CFU assay setup using 35mm dishes for custom hematopoietic training courses -
Courteney LaiCourteney Lai describes her work in the Humphries' lab studying leukemic stem cell function using models of acute myeloid leukemia -
Daniel Gray, PhDDr. Daniel Gray describes his work studying cell death and the potential applications in drug development for immune disorders -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Serum-Free Expansion of CD34+ Cells with SFEM II and UM729Explore the use of StemSpan™ SFEM II medium, StemSpan™ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs -
Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping PanelsTechnical tip from our dedicated team of Product and Scientific Support specialists
