Hybridoma and Mammalian Cell Line Development
Hybridomas, CHO cells and other mammalian cell lines are used for recombinant antibody and protein production. Generating stable cell lines which correctly and productively express the protein of interest is a lengthy and complex process. One of the most important considerations is achieving monoclonality of the cell line, while also encouraging robust cell expansion.
We've created these resources to help you accelerate your cell line development and get to production sooner.
Semi-Solid Cloning with ClonaCell™
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional cloning techniques. During semi-solid cloning, individual cells are immobilized within a viscous, semi-solid medium and grow into discrete, monoclonal colonies. Picking these colonies enables isolation of a large number of diverse clones with a high probability of monoclonality.
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How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCell™-CHO MediumThis video outlines the ClonaCell™-CHO 96-well plate semi-solid cloning protocol, including plating, liquid overlay, screening, and colony picking -
How to Generate Hybridomas in 96-Well Plates Using ClonaCell™-HY Semi-Solid Cloning MediumAdaptation of the ClonaCell™-HY semi-solid cloning protocol to a 96-well plate format -
Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO MediumProcedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion -
Biological Activity of Cytokines: Specific Activity (units/mg) vs. International Units (IU)Technical tip from our dedicated team of Product and Scientific Support specialists -
How to Prepare and Plate Semi-Solid MediumSteps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™) -
Considerations for Immunizing Mice for Hybridoma GenerationTechnical tip from our dedicated team of Product and Scientific Support specialists -
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO CellsCloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional cloning techniques -
ClonaCell™-HY ProcedureSelection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting
