Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, ϳԹ conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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Development of Simplified and Defined Cell Culture for Human Pluripotent Stem CellsHuman pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSR™-E8™ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau. -
Vitronectin XF™ Defined Matrix for Human Pluripotent Stem CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
mTeSR™1: Standardized Medium for the Feeder-Independent Maintenance of hESCs & hiPSCsHow mTeSR™1 simplifies and standardizes human embryonic & induced pluripotent stem cell cultures to minimize experimental variability -
The Role of Albumin in Feeder-Free Culture MediaDr. Hadley discusses using albumin in feeder-free culture media, and how it relates to mTeSR™1 and TeSR™-E8™ -
Transitioning Pluripotent Stem Cells from Feeder-Free Media to mTeSR™ PlusTransitioning human embryonic stem (ES) and induced pluripotent stem (iPS) cells from feeder-free media to mTeSR™ Plus, a serum-free, stabilized cell culture medium -
Enzyme-Free Passaging of Human Pluripotent Stem Cells Using Gentle Cell Dissociation ReagentHow to passage ES and iPS cells cultured in mTeSR™ Plus using GCDR
