Chimerism Analysis
Lineage-specific chimerism analysis is an important tool for monitoring the outcome of allogeneic hematopoietic cell transplantations (allo-HCT). Depending on engraftment success, the host may either achieve full donor chimerism or reach a state of mixed chimerism, in which host hematopoiesis persists alongside donor hematopoiesis in the recipient. Determination of the chimerism status in transplanted recipients can indicate the potential dynamics of disease relapse, graft-versus-host disease (GVHD), graft versus tumor (GVT) effects and other outcomes.
Below is a collection of scientific resources for your chimerism research.
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
This Technical Bulletin reviews the importance of lineage-specific chimerism analysis, explains the need for purity assessment, and provides guidelines for assessing purity using flow cytometry.
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Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
The Nuts and Bolts of Lineage Chimerism TestingChimerism analysis is used to monitor the outcome of allogeneic hematopoietic stem cell transplantation. By looking at the presence of donor leukocytes within specific cell subsets of the host’s peripheral blood, one can assess the success of donor cell engraftment or detect early graft failure, rejection, or relapse. Prior to performing chimerism analysis, different cell subsets must be isolated from the same sample to perform lineage-specific analysis. In this webinar, Dr. Cathi Murphey (Immunogenetics Lab Director; Southwest Immunodiagnostics, Texas) will discuss available technologies for isolating different cell subsets from the same sample and improving cell purity prior to chimerism analysis. -
Automate Cell Isolation with the RoboSep™-S Cell Separation InstrumentStreamline your cell isolations using the fully automated RoboSep™-S
