Hybridoma and Mammalian Cell Line Development
Hybridomas, CHO cells and other mammalian cell lines are used for recombinant antibody and protein production. Generating stable cell lines which correctly and productively express the protein of interest is a lengthy and complex process. One of the most important considerations is achieving monoclonality of the cell line, while also encouraging robust cell expansion.
We've created these resources to help you accelerate your cell line development and get to production sooner.
Semi-Solid Cloning with ClonaCell™
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional cloning techniques. During semi-solid cloning, individual cells are immobilized within a viscous, semi-solid medium and grow into discrete, monoclonal colonies. Picking these colonies enables isolation of a large number of diverse clones with a high probability of monoclonality.
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How to Generate Hybridomas in 96-Well Plates Using ClonaCell™-HY Semi-Solid Cloning MediumAdaptation of the ClonaCell™-HY semi-solid cloning protocol to a 96-well plate format -
ClonaCell™-HY ProcedureSelection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting -
Biological Activity of Cytokines: Specific Activity (units/mg) vs. International Units (IU)Technical tip from our dedicated team of Product and Scientific Support specialists -
How to Prepare and Plate Semi-Solid MediumSteps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
