Immuno-Oncology
Immune cells have the capability to eliminate tumor cells; however, tumor cells can develop the ability to evade immune responses. Current research efforts have been focused on developing personalized strategies for cancer treatment and developing new therapies that bolster the immune response in the fight against tumor cells.
Below is a collection of scientific resources for your immuno-oncology research.
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Production of Chimeric Antigen Receptor T Cells
This free Nature Protocols Wallchart summarizes the processes involved in producing CAR T cells for therapy.
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Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySep™ and EasyPlate™ EasySep™ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using EasySep™ Positive Selection Kits for Cell DepletionThe EasySep™ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
Acquiring DNA or RNA from Positively Selected CellsCells positvely selected with the EasySep™ system do not interfere with most downstream applications -
Sara McKee, PhDDr. Sara McKee discusses her research on antibody-based immunotherapy for B cell lymphoma -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Natural Killer CellsOverview of NK cell receptors, subsets, activation and function
