Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaTox™ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCult™ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Expansion of CD34+ Cells in Serum-Free CultureTechnical tip from our dedicated team of Product and Scientific Support specialists -
Maintaining Humidity in CFU Assays using MethoCult™ MediaTechnical tip from our dedicated team of Product and Scientific Support specialists -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Culture and Analysis of Hematopoietic Progenitor Cells in CFU AssaysUse these tips to better understand how the right medium can make the analysis of your hematopoietic CFU assays clearer -
Serum-Free Expansion of CD34+ Cells with SFEM II and UM729Explore the use of StemSpan™ SFEM II medium, StemSpan™ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs -
Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping PanelsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Modeling Alzheimer's risk using human TREM2-knockout microgliaAmanda McQuade from Dr. Mathew Blurton-Jones’s lab discusses her protocol for differentiating microglia from induced pluripotent stem cells (iPSCs) and the use of these microglia in vivo and in vitro to uncover the mechanisms of immune activation and neurodegeneration in Alzheimer’s disease. -
Extending the Life of a CFU AssayTechnical tip from our dedicated team of Product and Scientific Support specialists
