Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute ProtocolEasy and rapid procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow using the unique SepMate™ PBMC isolation tubes -
Automate Cell Isolation for Up to 16 Samples with the RoboSep™-16 Cell Separation InstrumentFeatures and benefits of using the fully automated cell isolation platform RoboSep™-16 which allows you to efficiently isolate cells from up to 16 samples simultaneously -
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)How to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation to enable cell separation by minimizing additional cell loss and improving the labeling of target cells -
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver MagnetsHow to isolate highly purified immune cells directly from whole blood and takes as little as 20 minutes from start to finish -
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell IsolationEasySep™ technology, a fast and easy immunomagnetic cell separation technology that allows you to isolate virtually any cell types from a variety of sample sources in as little as 8 minutes -
Loading the RoboSep™-S Carousel and Starting a RunBasic overview on how to use and maintain your RoboSep™-S; steps to load the instrument with samples and reagents and start a cell separation run -
Selecting a Protocol on the RoboSep™-SBasic overview on how to use and maintain your RoboSep™-S; steps involved to select a cell separation protocol -
Isolate Virtually Any Cell Type Using EasySep™ or EasySep™ Release Indirect Selection KitsUse any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySep™ reagents to obtain highly purified cells -
Importance of Using an FcR Blocker During Cell Separation ProceduresEasySep™ Other Species indirect selection kits can be used to isolate cells from any species -
How to Isolate Cells with EasySep™ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes -
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation TechnologyLearn how to perform the EasySep™ pour-off -
Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists
