Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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Acquiring DNA or RNA from Positively Selected CellsCells positvely selected with the EasySep™ system do not interfere with most downstream applications -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Automate Cell Isolation with the RoboSep™-S Cell Separation InstrumentStreamline your cell isolations using the fully automated RoboSep™-S -
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell SeparationDescribes statistical models that can be used to estimate the probability of monoclonality for limiting dilution cloning and semi-solid cloning -
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride LysisTechnical tip from our dedicated team of Product and Scientific Support specialists -
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver MagnetsHow to isolate highly purified immune cells directly from whole blood and takes as little as 20 minutes from start to finish -
Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySep™ and EasyPlate™ EasySep™ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Reducing Platelet Contamination in Blood SamplesPlatelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation -
Isolate Virtually Any Cell Type Using EasySep™ or EasySep™ Release Indirect Selection KitsUse any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySep™ reagents to obtain highly purified cells -
The Easy 50 EasySep™ Magnet: Portable Large-Volume Cell Separation In 25 MinutesLearn to use the portable yet powerful Easy 50 EasySep™ magnet to obtain purified cells from large volumes & a variety of sources -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ MagnetEasyEights™: Faster and Easier Multiple Sample Cell Isolations
