Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, ϳԹ conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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Reporting Practices for Publishing Results with hPSCsLearn how to plan and conduct your human pluripotent stem cell (hPSC)-based research following the ISSCR’s Standards for Human Stem Cell Use in Research -
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will cover maintenance and morphological assessment of hPSC cultures in mTeSR™ Plus -
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will cover the generation of cell aggregates and passaging protocol -
iPSCs As Models, Part 1: What Are Induced Pluripotent Stem Cells and How Do You Use Them?Hear from Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital, as she provides some introductory examples of how iPSCs are used for disease modeling and walks through how to generate and characterize iPSCs. Learn about quality control measures to improve reliability and reproducibility of data, and how to address interline variability in your experiments. -
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will provide step by step instructions for plate coating -
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free MediaProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus -
Quality by Design: Reagents and Support for hPSC-Derived Cell and Gene TherapiesEach year, more human pluripotent stem cell-derived (hPSC-derived) therapies move towards the clinic, and ϳԹ is committed to supporting researchers from discovery to clinical application. In this Innovation Showcase from ISSCR 2021, Lynn Csontos will demonstrate how we build quality into our products, processes, and policies to support your project timing, product quality, and overall vision. Kimberly Snyder will then present data that shows how TeSR™-AOF, a novel animal origin-free (AOF) hPSC maintenance medium, can support optimized cell quality, improved performance and reproducibility across all cell lines. -
Derivation and Applications of Human Pluripotent Stem CellsOverview of the derivation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) -
Enzyme-Free Passaging of Human Pluripotent Stem Cells Using ReLeSR™How to passage ES and iPS cells cultured in mTeSR™ Plus using ReLeSR™
